Fig. 1.

Differential sensitivity to CD62L antibody in vitro. (A) CLL PBMCs were cultured in the presence of anti-human CD62L antibody (DREG56; 0.1 μg/ml) and cell survival examined after 7 days using trypan blue exclusion. CLL cell survival was normalized to untreated CLL PBMCs survival. (B) CLL PBMCs were cultured for 7 days before non-adherent cells were removed by gentle agitation and MDCs were visualized by Giemsa staining (magnification = 20 ×). A representative image from one sensitive and one resistant patient is shown.