Fig. 1.

Schematic representation of the experimental design for the microarray analysis. (A) Normal donor CD14⁺ monocytes were co-cultured with human A375 melanoma cells for 68 h (MDSC induction). CD11b⁺ A375-MDSCs were isolated from the co-cultures by anti-CD11b⁺ microbead labeling and magnetic column separation (MDSC isolation). RNA for the microarray analysis was subsequently isolated from the purified A375-MDSCs; (B) 4-IPP (50 μM) was added during the A375:monocyte MDSC induction phase (A375-MDSC + 4-IPP), which was followed by the isolation of RNA from the purified 4-IPP-treated MDSCs; and (C) RNA was isolated from monocytes that were cultured for 68 h without tumor cells (cultured monocytes control).