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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: Mucosal Immunol. 2013 Aug 21;7(2):391–404. doi: 10.1038/mi.2013.58

Figure 1. Nod2−/− mice exhibit increased colonic permeability and increased in vivo IL-12p70 production, associated with an increased LP subpopulation of CD4+LAP+ T cells and enhanced in vivo TGF-β production.

Figure 1

A: measurement of colonic permeability by intrarectal administration of FITC-Dextran (see Methods). Data shown is derived from two separate experiments, each involving five mice/group; data represent mean±SE; B and C: In vivo IL-12p70 and IFN-γ production: cytokine content of protein extracted from lamina propria tissue was measured by ELISA; data represent mean±SE derived from 12 mice in each group; D: LPMCs isolated from colonic tissue were cultured for 72 h in the absence of stimulation (US) or in the presence of anti-CD3/28 stimulation, after which the culture supernatants were assayed for IFN-γ content by ELISA; data represent mean±SE derived from 10 mice in each group. Data were tested for statistical significance using Student’s t test. E: Percentage of LAP+ cells among CD3+CD4+ gated LP cells; data represent mean±SE derived from 12 mice/group; F: In vivo TGF-β production: TGF-β content of protein extracted from lamina propria tissue was measured by ELISA. Data represent mean±SE derived from 12 mice/group. Data were tested for statistical significance using Student’s t test.