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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: Mucosal Immunol. 2013 Aug 21;7(2):391–404. doi: 10.1038/mi.2013.58

Figure 2. Effect of ampicillin treatment (A) and ML-7 treatment (B,C) on the fraction of LP CD4+LAP+ cells among CD4+ LP cells.

Figure 2

A. Separate groups of mice were treated with ampicillin 200μg/100μl distilled water or 100μl distilled water alone (untreated) by oral gavage; for 14–21 days. Mice were sacrificed at 14 and 21 days of Ampicillin treatment, and at 21 days of water treatment, respectively. LP cells from colons isolated, stained for CD3, CD4 and LAP and analyzed by flow cytometry. Each column represent mean±SE * =p<0.05. Nod2−/− mice untreated (distilled water only) vs Ampicillin-treated 21 days by Student’s t test. B. Separate groups of mice were treated for 4 days with ML-7 twice a day i.p (see methods). At the end of treatment mice were intrarectally administered FITC-Dextran (see methods). Columns represent mean±SE of three mice/group. C. Separate groups of mice were treated for 4 days with ML-7 twice a day i.p (see methods). At the end of treatment mice were sacrificed, colons were collected and isolated LP cells stained for CD3, CD4 and LAP and analyzed by flow cytometry. Columns represent mean±SE of data from three different experiments. * =p<0.05. Nod2−/− mice untreated vs ML-7 treated mice by Student’s t test.