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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: Mucosal Immunol. 2013 Aug 21;7(2):391–404. doi: 10.1038/mi.2013.58

Figure 6. Nod2−/− mice exhibit less severe TNB-colitis compared with Nod2+/+ mice.

Figure 6

Mice were administered intra-rectal ethanol/TNBS to induce TNBS-colitis or ethanol alone. A. Body weight changes; Each point represents mean ±SE weight at indicated times expressed as percentage of day 0 weight and derived from three different experiments in which five mice/group were evaluated; * p<0.05 ethanol/TNBS-treated Nod2−/− vs. Nod2+/+ ; B. histological appearance of representative colonic tissue of TNBS-treated mice sacrificed at day three after TNBS administration; H.&E. staining, 40× magnification: a, Nod2+/+: severe, diffuse ulcerative colitis with dense transmural inflammatory cell infiltration of the submucosa, the smooth muscle and the serosa layers. b, Nod2−/− mild, non-ulcerative colitis with mild submucosal edema and mild inflammatory cell infiltration; Colitis score *p<0.05 ethanol/TNBS-treated Nod2−/− vs. Nod2 +/+ by Student’s t test. C. and D. in vitro IFN-γ and IL-17 production by unstimulated and anti-CD3/CD28-stimulated LPMC; data represent mean±SE of pooled values derived from three different experiments in which five mice/group were evaluated. Data were tested for statistical significance using one way analysis of variance with Bonferroni’s multiple comparison test.