Figure 8.

Mice were administered intra-rectal ethanol/TNBS to induce TNBS-colitis in untreated Nod2+/+ mice or in Nod2+/+ mice adoptively transferred LPMCs or LAP-depleted LPMCs from ethanol-treated Nod2−/− mice. A: Body weight changes; each point represents mean ±SE weight at indicated times expressed as percentage of initial weight; these data were from 2 experiments with eight mice/group except for LAP-depleted LPMC recipient group in which 5 mice were evaluated;*p<0.05 TNBS-colitis in Nod2+/+ recipients of Nod2−/− LPMC vs. Nod2+/+ mice with TNBS-colitis and Nod2+/+ mice recipient of LAP-depleted Nod2−/− LPMC B: histological appearance of representative colons of different groups of mice sacrificed at day 3 after TNBS administration; H&E staining, 40× magnification: a, severe, diffuse, transmural ulcerative acute colitis with severe submucosal inflammatory infiltration deeply involving the smooth muscle and serosa layers; b, moderate submucosal oedema and moderate inflammatory infiltration ; c, severe, diffuse, transmural ulcerative acute colitis with severe submucosal inflammatory infiltration deeply involving the smooth muscle and serosa layers ;d, normal architecture/patterns with small lymphoid nodules. Colitis score: ;*p<0.05 TNBS-colitis in Nod2+/+ recipients of Nod2−/− LPMC vs. Nod2+/+ mice with TNBS-colitis and Nod2+/+ mice recipient of LAP-depleted Nod2−/− LPMC. Data were tested for statistical significance using one way analysis of variance with Bonferroni’s multiple comparison test.