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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2936–2950. doi: 10.1002/jcp.25022

Figure 2. Primary structure analysis and nuclear localization of Nup1L protein.

Figure 2

(a) Pairwise alignment of the Nupr1 and Nupr1L proteins displaying a 60 % of homology. Residues labeled with a (*) indicate 100% identity, residues labeled with (:) indicate positions with conservative substitutions, residues labeled with (.) indicate substitutions less conservative. (b) Schematic representation of Nupr1L protein, which contains a Nuclear Localization Signal (NLS). The Flag-Nupr1L immunofluorescence in MiaPaca-2 (c) and Hela cells (d) reveals its nuclear localization. The cells were transduced with a lentiviral vector (6His-Flag-Nupr1L) and were plated on coverslips. The mouse Flag was revelated using Alexa fluorantimouse IgG488. Nuclei are stained by blue fluorescent DAPI.