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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2936–2950. doi: 10.1002/jcp.25022

Figure 3. Nupr1L gene is regulated by p53 protein.

Figure 3

(a) Diagram of the possible binding sites of different transcription factors in the Nupr1L promoter whose sequence is illustrated since the position -643 to +3 considering +1 the translation start site. Reporter gene assay for Nupr1L promoter in response to increasing concentration of pcDNA3-p53 in Panc-1 (b), MCF-7 (c) and Hela cells (d). The region of the Nupr1L promoter that was cloned into pGL3-luciferase vector (pGL3-Nupr1L-Luc) was comprised by the sequence since -245 to +93 position. Values are expressed as mean ± S.D. of triplicate, from 2 independent experiments. Means sharing the same superscript letter are not significantly different from one another (P< 0.05).