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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2936–2950. doi: 10.1002/jcp.25022

Figure 4. Nupr1L promoter contains two p53 regulation functional sites.

Figure 4

(a) Schematic representation of the different constructions of Nupr1L promoter that were cloned into pGL3-luciferase vector. WT, Nupr1L promoter sequence wild type; ΔS1, Nupr1L promoter sequence with the deleted sequence1 by directed mutagenesis; ΔS2 Nupr1L promoter sequence with the deleted sequence2 by directed mutagenesis; ΔS1+ΔS2, Nupr1L promoter sequence with both deleted sequences (1+2) by directed mutagenesis. The yellow band indicates the start codon. (b) Reporter gene assay for the different constructions of Nupr1L promoter (WT, ΔS1, ΔS2 and ΔS1+ΔS2) in Panc-1 cells, MCF-7 (c), and Hela cells (d). The bar denominated as control is a representation of the different controls corresponding to each construction. Values are expressed as mean ± S.D. of triplicate, from 2 independent experiments. Differences are expressed respect to the control; **P<0.01 and ***P<0.001; (NS) not significant differences.