Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2936–2950. doi: 10.1002/jcp.25022

Figure 7. Nupr1L down-regulates Nupr1 expression.

Figure 7

MiaPaca-2 cells were infected by 6His-Flag-Nupr1L lentiviral vector. (a) By RT-qPCR, decrease of Nupr1 transcript is observed in cells overexpressing Nupr1L. Western Blot shows the increase of NUPR1 protein in transduced cells with the lentiviral 6His-Flag-Nupr1L vector. (b) Reporter gene assay for Nupr1. Significant decrease in the activity was observed when cells were co-transfected with the 6His-Flag-Nupr1L and pGL3-Nupr1-Luc. (c) Similar results were showed at level of NUPR1 promoter in presence of p53, displaying a significant decrease in the luciferase activity. Nupr1L depletion did not modify the effect of p53 on Nupr1 promoter activity. (d) RT-qPCR of Nupr1L showing the effectiveness of specific siRNA. Values are expressed as mean ± S.D. Means sharing the same superscript letter are not significantly different from one another. *p<0.05 and ***p<0.001.