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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2936–2950. doi: 10.1002/jcp.25022

Figure 9. Nupr1 decrease the Nupr1L expression under metabolic stress conditions.

Figure 9

Expression of Nupr1L or Nupr1 by RT-qPCR in MiaPaca-2 cells (a) Nupr1L expression in transduced cells with 6His-Flag-Nupr1 lentiviral vector and starved of glucose for 24 h. GFP-overexpressing cells and cultured in conditioned medium were used as control. (b) Nupr1L expression in cells treated with siNupr1 or siControl and cultured under glucose starvation conditions for 24 h. (c) RT-qPCR of Nupr1 showing the effectiveness of specific siRNA. (d) Increase of Nupr1 expression under glucose starvation conditions. Conditioned medium was used as mock treatment. Values are expressed as mean ± S.D. Means sharing the same superscript letter are not significantly different from one another. *p<0.05 and ***p<0.001.