Overexpression of TIPE2 enhanced susceptibility to DR5-mediated apoptosis.
Notes: (A) Growth inhibition of AA-FLSs. The cells were stimulated with the indicated amounts of plate-bound ZF1 for 12 hours or 0.1 mg/mL of matrine for different time periods (0, 4, 8, 12, 24, and 48 hours) prior to the MTT assay. Mouse IgG (concentration 0.0125–0.2 mg/mL) on FLSs was used as the homotype control. The data shown are the mean from three parallel experiments (mean ± SD), ***P<0.001 vs the IgG group. (B) Flow cytometry detection of apoptosis with annexin V/PI in AA-FLSs stimulated with ZF1 at concentrations of 0, 0.025, 0.05, and 0.1 mg/mL. (C) The percentages of apoptotic cells (Q2 + Q3) (shown in Figure 2B) are presented as the mean ± SD of three independent experiments, **P<0.01; ***P<0.001.
Abbreviations: AA, adjuvant arthritis; DR, death receptor; FLS, fibroblast-like synoviocyte; MTT, 20 μL of a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide; PI, propidium iodide; SD, standard deviation; DR, death receptor; APC, allophycocyanin; IgG, intravenous gamma globulin.