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Bloodstream trypanosomes (3 x 103 cells/mL) were treated for 48 hours at varying concentrations of tideglusib (in DMSO solvent, final concentration of DMSO was 0.1%). Percent growth was determined by comparison to trypanosomes treated with equivalent volume of solvent (0.1% DMSO). Non-linear regression fit of the dose-response curve was determined using GraphPad Prism 6 software.
