Fig 9. Trypanosome viability after short term exposure with tideglusib.

Trypanosomes (5 x 10⁵/mL in HMI-9 medium) were treated with pentamidine (or water solvent control) or tideglusib (5 μM, 10 μM, or DMSO solvent control) for 6 hours (37°C, 5% CO₂) and then cultured in drug-free treatment as detailed in panel (A). Trypanosome densities were determined after 6 hour treatment (B), and the drugs washed from the cells by centrifugation and resuspension in fresh medium (to a density of 0.5 x 10⁵/mL). Trypanosome proliferation in the fresh medium was monitored for 44 hours (37°C, 5% CO₂) (C). Error between four separate cultures are shown. Cultures in which no cells were observed is denoted by an asterisk.