Fig 5. Identification of differentially expressed genes between NIL 16-PL and 16-GL seedlings under low temperature stress.

Five-leaf old NIL PH and GH seedlings grown at 28°C were treated at 28°C/20°C (day/night, 16h/8h, named 28-PL and 28-GL, respectively) or 16°C/8°C (day/night, 16h/8h, named 16-PL and 16-GL, respectively) for 5 days. RNA was extracted from leaves of individual lines and used for RNA-seq profiling. Two biological replicates of all samples were prepared. (A) The relative expression pattern of differentially expressed genes between 16-PL and 16-GL seedlings under low temperature conditions, excluding the differentially expressed genes from the 28-PL and 28-GL comparison. The genes in red color mean that the same expression pattern of these genes were observed in NIL-PH relative to NIL-GH. (B) The relative expression pattern of anthocyanin regulator genes AH, SlAN2, SlANT1, SlANT1-like and SlAN2-like in all samples. The correlation value of SlAN2, SlANT1-like to AH was evaluated by the normalized RPKM (log₂(RPKM+0.001)) from the 6 samples, respectively.