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. 2016 Mar 4;11(3):e0151083. doi: 10.1371/journal.pone.0151083

Fig 1. IL-17 and Th17 responses in the lungs and peripheral blood of calves infected with BRSV.

Fig 1

Calves (n = 8) were infected via aerosol inoculation with BRSV Strain 375 (RSV) as described in Materials and Methods. Control calves remained uninfected (n = 8). On day 7 post-infection, the animals were sacrificed and the lungs analyzed by qPCR for expression of IL-17, IL-21 and IL-22 (A) and IL-8 (B). In separate experiments, PBMC were isolated from control (n = 8) and BRSV infected calves (n = 8) on day 7-post infection. PBMC were stimulated with BRSV for 24 hours and then analyzed for expression of IL-17 by qPCR (C); or for 6 days and then cell culture supernatants were analyzed by ELISA for expression of IL-17 (D). For qPCR analysis, results were normalized to the housekeeping gene RPS-9, and expressed relative to samples from uninfected control calves. Results were pooled from a total of three independent experiments. Data represent means ± SEM.