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. 2016 Mar 4;11(3):e0151083. doi: 10.1371/journal.pone.0151083

Fig 3. Cell culture supernatants from BRSV stimulated PBMC induce expression of IL-8, MUC5B and MUC5AC by BT cells.

Fig 3

PBMC from control (nonvaccinated) and BRSV vaccinated cows were isolated and stimulated with heat-killed BRSV for 6 days as in Fig 2. Cell culture supernatants were then diluted 1:1 in cMEM and added to confluent BT cells in 96 well plates for 24 hours. RNA was the isolated from the BT and analyzed by qPCR for expression of IL-8 (A), MUC5B (B) and MUC5AC (C). For qPCR analysis, results were normalized to the housekeeping gene RPS-9, and expressed relative to unstimulated control samples. Results were pooled from two independent experiments. Data represent means ± SEM.