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. 2016 Mar 4;11(3):e0151083. doi: 10.1371/journal.pone.0151083

Fig 5. WC1.1+ γδ T cells produce IL-17 in response to BRSV.

Fig 5

γδ T cells were purified from the peripheral blood of BRSV vaccinated or control animals by FACS based upon their expression of the γδ T cell receptor and either expression of WC1.1, WC1.2 or lack of WC1. Cells were cultured in the presence of autologous APC ± BRSV for 6 days. Cell culture supernatants were then analyzed by ELISA for IL-17. (B) Cell culture supernatants from (A) were also diluted 1:1 and added to BT cells for 24 hours. After 24 hours, BT were analyzed by qPCR for expression of IL-8. For qPCR analysis, results were normalized to the housekeeping gene RPS-9, and expressed relative to unstimulated control samples. Results are pooled from two independent experiments. Data represent means ± SEM.