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. 2016 Mar 4;11(3):e0151083. doi: 10.1371/journal.pone.0151083

Fig 7. In vitro infection with BRSV and M. haemolytica results in exacerbated IL-17 production.

Fig 7

PBMC from BRSV vaccinated cows were stimulated with BRSV for 6 hours, then cultured with 0.1 MOI M. haemolytica in antibiotic free media for 4 hours. After four hours, antibiotics were added, and then incubations were continued for 18 hours (A-C) or 6 days (D and E). Control cultures were included that received neither pathogen, BRSV alone or M. haemolytica alone. (A-C) Cells were analyzed by qPCR for expression of IL-17 (A), IL-21 (B) and IL-22 (C). Cell culture supernatants from day 6 were analyzed by ELISA for IL-17 (D) and were diluted 1:1 and added to BT for 24 hours (E). BT were analyzed by qPCR for expression of IL-8 after overnight stimulation as in Fig 3. Results in A-C are pooled from three independent experiments with n = 12. Results in D and E are from two experiments with n = 8. Data represent means ± SEM.