Table 2. Primer sequences and expected amplicon lengths to detect the presence of specific viruses.

Virus	Forward Primer Sequence (PCR Reaction Concentration)	Reverse Primer Sequence (PCR Reaction Concentration)	Expected Amplicon Length (bp)	Ref.
Adenovirus	5'-CTGATGTACTACAACAGCACTGGCAACATGGG-3' (500 nM)	5'-GCGTTGCGGTGGTGGTTAAATGGGTTTACGTTGTCCAT-3' (500 nM)	605	[77]
Enterovirus-71	5'-ATAATAGCAYTRGCGGCAGCCCA-3' (500 nM)	5'-AGCTGTGCTATGTGAATTAGGAA-3' (500 nM)	317	[78]
IAV	5'-AAGACCAATCCTGTCACCTCTGA-3' (200 nM)	5'-CATTCTGTTGTATATGAGGCCCAT-3' (200 nM)	262	[36]
RSV	5'-TTGGATCTGCAATCGCCA-3' (300 nM)	5'-CTTTTGATCTTGTTCACTTCTCCTTCT-3' (300 nM)	75	[79]
Rhinovirus	5'-TGGACAGGGTGTGAAGAGC-3' (200 nM)	5'-CAAAGTAGTCGGTCCCATCC-3' (200 nM)	235	[80]
Rotavirus	5'-GCTATTAAAGGCTCAATGGCGTAC-3' (250 nM)	5'-GGATGTAGAATTGATGGATAATTG-3' (250 nM)	834	[81]