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. 2016 Mar 4;11(3):e0150792. doi: 10.1371/journal.pone.0150792

Fig 4. Islet resident macrophages possess an immunoregulatory phenotype that is compromised by TLR4 activation.

Fig 4

(A) IRMs (solid line) and IRDCs (dashed line) were analyzed directly ex vivo by flow cytometry for CD39, CD73 and galectin-9 using the CD45+Ly6C-CD11c+F4/80+CD16/32+ gate. Shaded histograms are the FMO controls and MFIs are indicated parenthetically. Representative data are shown from one of three independent experiments. (B) FACS-sorted B6 IRMs were stimulated with 1 μg/ml of LPS or 10 μg/ml of agonistic anti-CD40. After 72 h, mRNA from cultured cells was analyzed by real-time PCR for Entpd1 (CD39), Ccl2, Il1b, and Il6 transcripts. Each point represents data from an independent experiment (n = 3–4 per group).