Skip to main content
. 2016 Mar 4;10(3):e0004467. doi: 10.1371/journal.pntd.0004467

Fig 5. Endothelia cell (EC) activation-associated gene expression in the kidneys of infected mice.

Fig 5

WT mice (4-5/group) were inoculated with O. tsutsugamushi Karp strain as in Fig 1. (A) At 0, 2, 6, and 10 dpi, kidney tissues were analyzed for angiopoietin (Ang) 1 and Ang2 expression by qRT-PCR. Data are presented as “qPCR fold” (after normalization to the housekeeping genes), and are shown as mean ± SEM in each group. (B) The Ang2/Ang1 ratios of individual samples were calculated based on the qRT-PCR data and compared with mock controls (0 dpi). WT and IL-33-/- mice (4-5/group) were infected and euthanatized at 9 dpi. Kidney tissues were analyzed for the expression of Ang1, Ang2 (C), Ang2/Ang1 ratio (D), endothelial nitric oxide synthase (eNOS), Endothlin-1 (ET-1) (E), and eNOS/ET-1 ratio (F), respectively. *, p < 0.05; **, p < 0.01; NS, no significance.