Table 3. Stem-loop RT-qPCR confirmation for the significantly differentially-expressed miRNAs.
| Mature miRNA ID | Pre-miRNA ID | qRT-PCR fold-change (Infected/control) | High throughput sequencing fold-change (Infected/control) |
|---|---|---|---|
| ssc-miR-101 | ssc-mir-101-1 | +1.48 | +18.60 |
| ssc-miR-7 | ssc-mir-7-2 | +2.69 | +7.60 |
| ssc-miR-128 | ssc-mir-128-1 | +1.55 | +4.97 |
| ssc-miR-155-5p | ssc-mir-155 | +3.03 | +19.67 |
| ssc-miR-196b-5p | ssc-mir-196b-1 | +1.36 | +3.56 |
| ssc-miR-novel-chr6_30729 | ssc-mir-novel-chr6_30729 | +1.51 | +2.02 |
| ssc-miR-18a | ssc-mir-18a | -0.34 | -0.13 |
| ssc-miR-19b | ssc-mir-19b-2 | -0.98 | -0.43 |
| ssc-miR-24-3p | ssc-mir-24-1 | -0.29 | -0.01 |
| ssc-miR-novel-chr5_29857 | ssc-mir-novel-chr5_29857 | -0.44 | -0.015 |
Comparison of the levels of miRNA expression detected by qRT-PCR and high-throughput sequencing. “+” and “−” indicate upregulated and downregulated miRNAs, respectively. The fold change cutoffs of the upregulated miRNAs and the downregulated miRNAs were 2 and 0.5, respectively. qRT-PCR Ct threshold is 0.015.