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. 2016 Mar 4;12(3):e1005868. doi: 10.1371/journal.pgen.1005868

Fig 2. Non-canonical components are required for sex-induced silencing via RNAi.

Fig 2

(A) Deletion of ZNF3 and QIP1 significantly reduces silencing in unilateral mutant matings. Progeny from wild type and unilateral mutant matings (one parent is mutant) were isolated and evaluated for URA5 silencing by growth on rich media (YPD) and SD-uracil. The parents and ura5 mutant were included as controls. The plates were incubated at 30°C for 3 days. Strains used: H99α crossed with JF289a and H99α crossed with XW205. (B) Schematic indicating the presence or absence of the transgene array in the spores from (A) as tested by PCR (Primary data in S3 Fig), as well as the occurrence of silencing. (C) Frequency of sex-induced silencing in all mutants tested. Primary data is shown in S4 Fig. The asterisk indicates that only unilateral silencing was tested for qip1Δ mutants because bilateral matings did not produce viable spores. Strains used: for znf3Δ: MF65 and XW205; for qip1Δ: SEC1, SEC2, SEC3, SEC4, and H99; for cpr2Δ: YPH16, XW197, JF289a; for fzc28Δ: SEC7, SEC8, YSB2337, YSB2338, H99; and for fzc47Δ: SEC5, SEC6, YSB1406, YSB1407, and H99.