Skip to main content
. 2016 Mar 4;12(3):e1005868. doi: 10.1371/journal.pgen.1005868

Fig 3. Non-canonical components are required for mitotic-induced silencing via RNAi.

Fig 3

Znf3 and Qip1 are required for URA5 repetitive transgene silencing during vegetative growth. Wild type JF289a isolate and the derived deletion mutants were grown in rich media and plated on 5-FOA to calculate the frequency of URA5 silencing. The silencing frequency is normalized to the frequency of the wild type JF289 strain and plotted with error bars indicating the standard deviation from the mean. The other three mutants tested showed only minor non-significant variation in silencing rate from the wild type. Strains used: JF289a (wild type), XW205 and XW206 (znf3Δ), SEC1 and SEC2 (qip1Δ), XW197 and XW198 (cpr2Δ), SEC7 and SEC8 (fzc28Δ), and SEC5 and SEC6 (fzc47Δ).