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. 2016 Mar 4;14(3):e1002401. doi: 10.1371/journal.pbio.1002401

Fig 1. A common cysteine substitution for tyrosine in the Fas death domain in primates and rodents and site-directed mutagenesis suggest that the tyrosine phosphorylation is dispensable for Fas apoptosis.

Fig 1

(A) Partial sequence alignment of Fas death domain (helices 1 and 5 [9]) in vertebrates. Positions 232 and 291 of human Fas are highlighted. Cysteine and alanine at the 291 position of Fas are boxed in red. (B) SW480 cells stably expressing control protein (LacZ.V5) or indicated Fas.V5 proteins were treated with 10 ng/ml FasL crosslinked with anti-FLAG (M2) for 24 h and subsequently subjected to DNA content analysis by flow cytometry. Numbers indicate percentage of cells that underwent apoptosis, having subG1 DNA content due to DNA fragmentation.