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. 2016 Mar 3;95(8):e2697. doi: 10.1097/MD.0000000000002697

FIGURE 2.

FIGURE 2

TDO2, CTNNB1, and CSNK1A1 were validated as synthetic lethal to APC. A, B, Knockdown of TDO2, CTNNB1, or CSNK1A1 inhibited the proliferation of APC mutant cells SW620, not APC wild-type cells SW480. SW480, and SW620 cells were transiently transfected with control siRNA or siTDO2, siCTNNB1, and siCSNK1A1 for 3 days or indicated time points followed by MTT assay or CCK8 assay. C, The siRNA knockdown effects were confirmed by RT-PCR. D, E, 680C91 selectively inhibited proliferation of APC mutant cells. Colorectal adenocarcinoma SW480, SW620, and LoVo cells were treated with indicated concentrations of 680C91 for 3 days followed by MTT assay (D) or treated with 50 μM 680C91 for indicated time points followed by CCK8 assay (E). Data were expressed as mean ± S.E. ∗, P < 0.05; ∗∗, P < 0.01 (n≥4, the experiments were repeated at least twice).