Figure 4.

Shade‐grown seedlings are hypersensitive to perturbation of cMT dynamics (A) Hypocotyl width of wild type seedlings grown on 1/2MS supplemented with various concentrations of PPM under Wc and shade. Error bars represent standard error of mean (n = 20). (B) Responses of cMTs (visualized using GFP‐TUA6) to PPM in hypocotyl cells of seedlings (35S::GFP‐TUA6) grown in Wc and shade. 5‐d‐old light grown seedlings were treated with Wc or shade for 24 h, they were then soaked in 10 μM of PPM. The figures show representative pattern of GFP‐TUA6 in these seedlings in response to PPM treatment. (C) Responses of Col‐0, taa1, dwf4 and ga3ox1 to PPM in shade. Seedlings were grown on 1/2MS supplemented with various concentrations of PPM under shade. Hypocotyl width was measured. Error bars represent standard error of mean (n = 30). *Student's t‐test results indicate that there is no significant difference between control and PPM‐treated samples with P > 0.1. **t‐test results show there is significant difference between control and PPM‐treated samples with P < 0.001. The number below ** is the absolute difference in hypocotyl width (mm) between PPM treated and control samples. (D) Responses of cMTs (visualized using GFP‐TUA6) to PPM in hypocotyl cells in the presence of NPA. 35S::GFP‐TUA6 seedlings grown on 1/2MS medium supplemented with/without 2 μM of NPA for 5 d. They were then treated with Wc or shade for 24 h prior to PPM treatment. Scale bars=20 μm.