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. Author manuscript; available in PMC: 2017 Feb 27.
Published in final edited form as: J Mol Biol. 2015 Sep 4;428(5 Pt A):822–833. doi: 10.1016/j.jmb.2015.08.021

Fig. 3. 2D NOESY spectra show that the upper stem is more dynamic in the methylated than in the unmethylated M2577 hairpin.

Fig. 3

(a) Superimposed imino regions of the 2D 1H NOESY NMR spectra of M2577-A (blue) and M2577-m6A (red) in 10% D2O. The spectra of 0.47 mM RNA were measured at 4 °C with a 100 ms mixing time. (b) Superimposed imino regions of the 2D 1H NOESY NMR spectra of M2577-A and M2577-m6A in 10% D2O. The spectra of 1.12 mM RNA were measured at 20 °C with a 100 ms mixing time. (c) Separate and superimposed amino–imino regions of the 2D 1H NOESY NMR spectra of M2577-A and M2577-m6A in 10% D2O at 20 °C. (d) Superimposed H6/H8–H1′ regions of the 2D 1H NOESY NMR spectra of M2577-A and M2577-m6A in 100% D2O. The spectra of 0.78 mM RNA were measured at 20 °C with a 100 ms mixing time.