Fig. 1.

Immunizing mice of different H-2 haplotypes with MAG-Tn3 induce a specific anti-Tn IgG response associated with a TT-specific T cell response. a HLA-DR1*A2 transgenic mice were im immunized on days 0, 21, 42, 63 and 84 with 15 (n = 12), 45 (n = 12) or 60 μg (n = 10) of MAG-Tn3 with 1/20, 1/7 and 1/5 of the AS15 immunostimulant, respectively, or with the AS01B immunostimulant alone. b C57BL/6J, C3H/HeN, BALB/c mice (n = 6) and NMRI and CD-1 mice (n = 8) were im immunized on days 0, 21 and 42 with 15 μg of MAG-Tn3 with 1/20 of the AS15 immunostimulant (middle and right panels), or with the AS01B immunostimulant alone (left panel). Sera were collected on days 21, 28 and 49 and tested for Tn-specific IgG by ELISA using Tn3-G6K (Biot) G. Antibody titers are expressed as the mean of Log10 individual antibody titers ± SEM. c. IFN-γ, IL-5, IL-13 and IL-17 production was analyzed by ELISA on supernatants of splenocytes stimulated with (black bars) or without (white bars) 50 μg/mL of TT for 72 h. The results are expressed as the means of individual mice ± SEM. The statistical significance of differences was determined by the Student t test (*P < 0.05, **P < 0.01). ND not detectable