Fig. 2.

The anti-Tn IgG response induced by the MAG-Tn3 vaccine is CD4⁺ T cell dependent. a, b C3H/HeN mice (n = 6/group) that were left untreated or treated with anti-CD4 or isotype control antibodies (300 μg ip) on days −1, 0, 1, 20, 21 and 22 were im immunized on days 0 and 21 with 15 μg of MAG-Tn3 with 1/20 of the AS15 immunostimulant. CD4⁺ T cell depletion was confirmed by FACS analysis of the percentages of CD4⁺ and CD8⁺ cells among CD3⁺ cells on blood samples collected on day 21, prior to vaccination. Dot plots of one representative mouse per group are shown (a). The results are expressed as the mean of individual mice ± SEM (b). c Sera were collected on days 21 and 28 and tested for Tn-specific IgG by ELISA, using Tn3-G6K (Biot) G. Antibody titers are expressed as the mean of Log10 individual antibody titers ± SEM. The statistical significance of differences was determined by the Student t test (**P < 0.01)