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. 2016 Feb 4;65(3):315–325. doi: 10.1007/s00262-016-1802-0

Fig. 3.

Fig. 3

The TT epitope can be recognized and presented by different murine MHC class II and human HLA-DRB1*01:01 molecules. ac. C3H/HeN mice (n = 5) were sc immunized with 50 μg of TT or HEL with CFA. On day 11, TNF-α production was analyzed by ICS on individual lymph nodes (a) and IFN-γ (b) and IL-2 (c) production was analyzed by ELISA on pooled lymph nodes stimulated with TT (black bars) or HEL (white bars). The results are expressed as the mean of individual mice ± SEM. d, e, f. HLA-DR1*A2 (n = 3), C57BL/6J (n = 3), BALB/c (n = 3), NMRI (n = 3) and CD-1 (n = 6) mice were sc immunized with 50 μg of TT or with immunostimulant alone on days 0 and 14. On day 28, TNF-α production was analyzed by ICS (d) and IFN-γ (e) and IL-2 (f) production was analyzed by ELISA on splenocytes stimulated with TT (black bars) or HEL (white bars). The results are expressed as the mean of triplicates ± SEM. The statistical significance of differences was determined by the Student t test (*P < 0.05, **P < 0.01)