Fig. 5.

Immunization of cynomolgus monkeys with the MAG-Tn3 vaccine induces a specific anti-Tn antibody response associated with a T cell response to TT. Cynomolgus monkeys (n = 10/group) were immunized on days 0, 21, 42, 63, 84 and 105 with NaCl, AS15 or MAG-Tn3 formulated with AS15. a Sera were collected on the day of each immunization and tested for Tn-specific IgM and IgG responses by ELISA. b, c T cell responses were measured on cynomolgus PBMCs harvested after five immunizations (day 105), stimulated with (black bars) or without TT (white bars). IFN-γ production was evaluated by ELISPOT and is expressed as the mean of individual SFC per 10⁶ cells ± SEM (b). Pictures are shown of one representative well per group, seeded with 10⁶ cells (c). The statistical significance of differences was determined by the Student t test (**P < 0.01)