Fig. 2.
Effect of AKR1B10 on the proliferation of NPC cells. a AKR1B10 protein expression was assayed in CNE-2/AKR1B10 and CNE-2/vector cells by western blot. b 5 × 103 or 7 × 103 CNE-2/vector and CNE-2/AKR1B10 cells per well were seeded into 96-well plates and cultured for 72 h. Cell proliferation was analyzed by MTT assay (*p < 0.05). c Cell cycle distribution was detected by propidium iodide staining and flow cytometry. i Representative cell cycle flow cytometry images of CNE-2/vector and CNE-2/AKR1B10 cells. ii Cell proliferation index (PI) was calculated according to the equation: PI = (S % + G2/M %)/(G0/G1 % + S % + G2/M %) × 100 %, and the PI of CNE-2/vector cells was higher than that of CNE-2/AKR1B10 cells (*p < 0.05). All the experiments were repeated three times at least