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. 2016 Mar 5;17:186. doi: 10.1186/s12864-016-2551-3

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© Ma et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Testing presence of the 1-kb transposon-like insertion in the 5’UTR of HvAACT1 associated with Al tolerance. Primers sets used in the PCR reactions are listed: (a) from Fujii et al. [23] (b) from Bian et al. [18] and (c) a new set of primers farther upstream and downstream from those used in (a) (see Methods). Varieties are Dayton (1), CXHKSL (2) and Gairdner (3)