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. 2016 Mar 5;7:16. doi: 10.1186/s40104-016-0074-z

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© Elkhenany et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Immunoblot analyses. Western blots showing the expression of OPN, BMP-7, p38 and p44/42 in low and high passaged cBMMSCs and cADMSCs. Autoradiographs of undifferentiated (lanes 1, 2, 3) and differentiated (lanes 4, 5, 6) cells at three time points, d 7, 14, 21 are shown. Graphs represent the relative expression of each protein in the differentiated samples normalized with that in the undifferentiated samples. β tubulin was used as a housekeeping control. Dotted line indicates the protein expression in undifferentiated cells normalized to 100. Significantly different values (P < 0.05) represent the comparison between the three time points within a specific group. Identical letters indicate no significance