Fig. 4.

NF-κB (p105/p50) expression and effect of ozone exposure. Left panel: a Representative Western blot images of NF-κB (p105/p50) expression in males and females with filter air and O₃ exposure; b univariate analysis of NF-κB (p105/p50) expression in males and females with FA and O₃ exposure; two-way ANOVA interaction effect of sex (c) and exposure (d) for NF-κB (p105/p50) expression. Right panel: e Representative Western blot images of NF-κB (p105/p50) expression in estrous cycle stages of females, with filter air and O₃ exposure; f univariate analysis of NF-κB (p105/p50) expression in estrous cycle stages of females with FA and O₃ exposure. Two-way ANOVA interaction effect of exposure (g) and estrous cycle stages (h) for NF-κB (p105/p50) expression. Univariate analysis data expressed as Ranks-Kruskal-Wallis test of densitometric analysis; the values are depicted as mean with SD, where **p ≤ 0.01 is the level of statistical significance compared to controls (n = 6–8 per group). Two-way ANOVA for NF-κB (p105/p50) expression analysis is given in Table 9