Fig. 5.

AKT1 expression and effect of ozone exposure. Left panel: a Representative Western blot images of AKT1 expression in males and females with filter air and O₃ exposure; b univariate analysis of AKT1 expression in males and females with FA and O₃ exposure; two-way ANOVA interaction effect of sex (c) and exposure (d) for AKT1 expression. Right panels: e Representative Western blot images of AKT1 expression in estrous cycle stages of females, with filter air and O₃ exposure; f univariate analysis of AKT1 expression in estrous cycle stages of females with FA and O₃ exposure. Two-way ANOVA interaction effect of exposure (g) and estrous cycle stages (h) for AKT1 expression. Univariate analysis data expressed as Ranks-Kruskal-Wallis test of densitometric analysis; the values are depicted as mean with SD where **p ≤ 0.01 and ***p ≤ 0.001 are the levels of statistical significance compared to controls (n = 6–8 per group). Two-way ANOVA for AKT1 expression analysis is given in Table 10