Table 4.
Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for STAT3 expression
Univariate tests: dependent variable: STAT3 | |||||||
df | F | Sig. | Partial η2 | ||||
Analysis of gender and exposure | Exposure | FA | 1, 52 | 0.031 | 0.862 | 0.001 | |
Ozone | 1, 52 | 4.220 | 0.045 | 0.075 | |||
Sex | F | 1, 52 | 0.152 | 0.698 | 0.003 | ||
M | 1, 52 | 2.217 | 0.143 | 0.041 | |||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | 1, 24 | 1.916 | 0.179 | 0.074 | |
Proestrus | 1, 24 | 16.113 | 0.001 | 0.402 | |||
Exposure | FA | 1, 24 | 0.087 | 0.770 | 0.004 | ||
Ozone | 1, 24 | 32.414 | < .0005 | 0.575 | |||
Pairwise comparisons: dependent variable: STAT3 | |||||||
(I) Sex | (J) Sex | Mean difference (I–J) | 95 % CI for difference | ||||
Lower bound | Upper bound | ||||||
Analysis of gender and exposure | Exposure | FA | F | M | 0.012 | 0.120 | 0.143 |
Ozone | F | M | 0.135 | 0.003 | 0.267 | ||
Sex | F | Ozone | FA | 0.026 | 0.106 | 0.157 | |
M | FA | Ozone | 0.098 | 0.034 | 0.230 | ||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | FA | Ozone | 0.143 | 0.070 | 0.356 |
Proestrus | Ozone | FA | 0.414 | 0.201 | 0.628 | ||
Exposure | FA | Proestrus | Non-proestrus | 0.030 | 0.183 | 0.244 | |
Ozone | Proestrus | Non-proestrus | 0.588 | 0.375 | 0.801 |