Table 3.

Reduction in the environmental impacts due to on-site enzyme production

Process configuration	Reduction of environmental impacts
	GWP [%]	EP [%]	AP [%]	POCP [%]
Base case	59	53	32	67
Highest yield at 20 % DM	62	68	44	77
Highest yield at 30 % DM	65	69	51	85

Impacts of on-site enzyme production were compared with the impacts of off-site enzyme production. This was done for the: a. base case (detoxification with ${\mathrm{Na}}_2{\mathrm{S}}_2{\mathrm{O}}_4$, PSSF at 12 % DM and 30 ${}^{\circ }$C); b. process configuration with the highest yield at 20 % DM (washing of slurry, PSSF at 40 ${}^{\circ }$C); c. process configuration with the highest yield at 30 % DM (adaptation of yeast + extra nutrients, SHF at 30 ${}^{\circ }$C)