Fig. 4.

The multiple sequence alignments of the RGD-disintegrin domain (cluster BC03, a representative clone JZ880095), ECD-disintegrin and Cys-rich domains (cluster BC05, a representative clone JZ880091, and cluster BC06, a representative clone JZ880094) of metalloproteinases predicted from partially sequenced clones from B. colombiensis with other homologous venom proteins. The major isoforms from each group is aligned with closely related protein in the database, and the  % identities are shown in the figure. The alignment was generated with the ClustalW multiple sequence alignment program with manual adjustment and displayed with shaded boxes. The numbers in parenthesis are the NCBI accession numbers. The asterisk above the sequences represent the tripeptide binding motif in PII (RGD) and in PIII (ECD). All cysteine residues (letter “C” above the sequences) are conserved except for an extra cysteine residue (the letter “C” highlighted in black) in JZ880095, JZ8800100, jararin, salmosin 3, and r-Cam-dis. The source of sequences were as follows: Jararin [Q0NZX6.1] from B. jararaca, r-Cam-dis [J9Z332.1] from C. adamanteus, salmosin-3 [O93515.1] from G. brevicaudus, viridistatin [AEY81222.1] from C. viridis viridis, mojastin 2 isolated from the venom of C. scutulatus scutulatus, the native disintegrin colombistatin [P18618.2] from B. colombiensis, MP_III3 SVMP [ADO21503.1] from B. neuwiedi, HF3 from B. jararaca, MP_III1 [ADO21501.1] from B. neuwiedi, leucurolysin-B [P86092.1] from B. leucurus, Halysase [Q8AWI5.1] from Gloydius halys, Crotastatin [Q076D1.1] from C. durissus terrificus, and Met VMP-III [ACV83929.1] from A. contortrix laticinctus