Table 2.
Relative abundances of putative toxin-encoding clusters identified in B. colombiensis venom gland transcriptome
| Clusters | No. ESTs | HIT ID | Annotation | E value |
|---|---|---|---|---|
| Metalloproteinases | ||||
| BC01 | 107 | P83512.2 | Snake venom metalloproteinase BaP1 [Bothrops asper] | 0.00E+00 |
| BC02 | 1 | ABP48735.1 | Nonhemorrhagic metalloprotease MP-II, partial [Bothrops jararacussu] | 9.80E−27 |
| BC03 | 2 | ADO21506.1 | MP_IIb1 SVMP precursor, partial [Bothrops neuwiedi] | 0.00E+00 |
| BC04 | 1 | Q7SZD9.1 | Zinc metalloproteinase/disintegrin ussurin [Gloydius ussuriensis] | 4.40E−21 |
| BC05 | 5 | Q98UF9.3 | Zinc metalloproteinase-disintegrin-like HF3 [Bothrops jararaca] | 0.00E+00 |
| BC06 | 7 | Q2LD49.1 | Zinc metalloproteinase-disintegrin-like TSV-DM [Trimeresurus stejnegeri] | 1.30E−75 |
| BC07 | 3 | C5H5D4.1 | Zinc metalloproteinase-disintegrin-like batroxstatin-3 [Bothrops atrox] | 4.00E−56 |
| BC08 | 1 | Q98UF9.3 | Zinc metalloproteinase-disintegrin-like HF3 [Bothrops jararaca] | 0.00E+00 |
| BC09 | 1 | Q8UVG0.1 | Zinc metalloproteinase-disintegrin-like berythractivase [Bothrops erythromelas] | 0.00E+00 |
| BC10 | 1 | C9E1S0.1 | Zinc metalloproteinase-disintegrin-like VMP-III [Agkistrodon piscivorus leucostoma] | 1.00E−14 |
| Total ESTs | 129 | |||
| PLA2s | ||||
| BC11 | 71 | P24605.3 | Basic phospholipase A2 homolog 2, myotoxin II [Bothrops asper] | 3.00E−88 |
| BC12 | 1 | P20474.2 | Basic phospholipase A2 myotoxin III [Bothrops asper] | 5.30E−13 |
| BC13 | 30 | 5.40E−82 | Acidic phospholipase A2 BmooPLA2 [Bothrops moojeni] | 5.40E−82 |
| Total ESTs | 102 | |||
| Serine proteinases | ||||
| BC14 | 39 | P04971.1 | Thrombin-like enzyme batroxobin [Bothrops atrox] | 3.50E−146 |
| BC15 | 2 | Q5W959.1 | Snake venom serine protease HS114 [Bothrops jararaca] | 5.90E−109 |
| Total ESTs | 41 | |||
| Waprin | ||||
| BC16 | 19 | BAN89446.1 | Waprin, partial [Ovophis okinavensis] | 1.0E−17 |
| Total ESTs | 19 | |||
| C-type lectins | ||||
| BC18 | 8 | P0C930.1 | Snaclec bothroinsularin subunit beta [Bothrops insularis] | 2.20E−49 |
| BC19 | 3 | BAN89423.1 | C-type_lectin_beta-subunit [Ovophis okinavensis] | 1.00E−12 |
| BC20 | 2 | BAP39929.1 | C-type lectin B subunit, partial [Protobothrops elegans] | 3.60E−38 |
| BC21 | 1 | BAP39964.1 | C-type lectin F IX/X B [Protobothrops flavoviridis] | 5.5E−79 |
| Total ESTs | 14 | |||
| ATPase | ||||
| BC22 | 9 | ACJ46370.1 | ATPase 6 [Agkistrodon piscivorus] | 2.70E−93 |
| BC23 | 1 | ETE73855.1 | V-type proton ATPase subunit e 1 [Ophiophagus hannah] | 9.70E−47 |
| Total ESTs | 10 | |||
| CRISP | ||||
| BC24 | 7 | BAP39957.1 | Cysteine-rich venom protein, partial [Protobothrops flavoviridis] | 1.10E−158 |
| BC25 | 1 | ETE59024.1 | Cysteine-rich protein 1 [Ophiophagus hannah] | 6.60E−49 |
| Total ESTs | 8 | |||
| svVEGF | ||||
| BC26 | 8 | Q90X24.1 | Snake venom vascular endothelial growth factor toxin [Bothrops insularis] | 3.60E−93 |
| Total ESTs | 8 | |||
| LAO | ||||
| BC27 | 6 | B5AR80.1 | L-amino-acid oxidase [Bothrops pauloensis] | 0.00E+00 |
| BC28 | 1 | X2JCV5.1 | L-amino-acid oxidase [Cerastes cerastes] | 5.30E−39 |
| Total ESTs | 7 | |||
| BPP/C-NP | ||||
| BC29 | 3 | BAP39952.1 | Bradykinin-potentiating and C-type natriuretic peptides, partial [Protobothrops flavoviridis] | 2.00E−09 |
| Total ESTs | 3 | |||
| Phospholipase B | ||||
| BC30 | 1 | BAN82026.1 | Phospholipase B [Protobothrops flavoviridis] | 3.70E−152 |
| BC31 | 1 | F8S101.1 | Phospholipase B [Crotalus adamanteus] | 3.60E−107 |
| Total ESTs | 2 | |||
| Phosphodiesterase | ||||
| BC32 | 1 | BAN89425.1 | Phosphodiesterase [Ovophis okinavensis] | 0.00E+00 |
| Total EST | 1 | |||