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. 2016 Mar 5;17:191. doi: 10.1186/s12864-016-2486-8

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© Williams et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of candidate pathogenicity genes in vitro versus in planta. a Expression of SIX genes and b candidate pathogenicity genes as determined by qRT-PCR in in vitro samples and M. truncatula DZA315 root samples harvested at 1, 2, 4 and 7 days post inoculation (dpi) with Fom-5190a. In vitro samples are averages ± SE of 3 biological replicates. In planta samples are averages ± SE of 4 biological replicates each consisting of pools of 10 seedlings. Gene expression levels are relative to the fungal actin gene (FOXM-5190a_13365). Note: ^ No detectable expression in 1 or 2 out of the 3 in vitro replicates