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EDTA inhibits self-assembly, but EGTA does not. (A) Time-lapse experiments assessing EDTA and EGTA compared with control conditions reveal significant impairment of self-assembly with EDTA. Scale bar 20 μm. (B) Quantification of the self-assembling process. While the presence of EGTA (when primarily cadherin is blocked) does not inhibit chondrocyte self-assembly, chondrocytes cannot self-assemble in the presence of EDTA (when both integrin function and cadherin function are blocked).
