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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2015 Feb 14;570:14–22. doi: 10.1016/j.abb.2015.01.028

Figure 7. BAH mediated release of heme b is specific to MPO.

Figure 7

Total protein-stained gel images (Panel A) and luminescence generated images (Panel B) for a molecular weight marker (lane 1), MPO alone (1.2 µM, lane 2), and reactions of MPO (1.2 µM) with 20 mM BAH in the presence of 20 µM H2O2 (lane 3), LPO (1.5 µM) alone (lane 4), and LPO (1.5 µM) with 20 mM BAH in the presence of 20 µM H2O2 (lane 5) at room temperature for 30 min. Experiments conducted as described under “Experimental Procedures.”