Figure 4.
Heart failure increases tPTP openings. (A) Characterization of transverse aortic constriction induced HF model in mouse. Heart morphology and function were determined by measuring heart size, echocardiography indices (B) Fractional shortening), heart weight/body weight, and lung weight/body weight ratios (**P<0.01, n=5 hearts; C). (D) Frequency of tPTP openings increased in HF, and could be inhibited by applying CsA. (1.09 ± 0.42 vs. 26.5 ± 5.9 vs. 3.74 ± 0.56, Sham, HF, HF+CsA, ***P<0.001 vs Sham, ##P<0.01 vs HF treatment, n=5-7 cells) (E) Amplitude of [Ca2+]mito rise in sham and HF during 10 min (n=7). (F) Peroxide generation in sham and HF during rest and 1Hz pacing (**P<0.01, n=10-11).