Fig. 7.

Effects of adult beta cell-specific Bmal1 deletion on GSIS in vitro in isolated islets. (a) Representative pulsatile and (b) mean ± SEM (n=5 per group) insulin secretion profiles sampled at 4 mmol/l (0–40 min) and 16 mmol/l glucose (40–80 min) in islets from β-Bmal1^+/+ (thin line, white circles) and β-Bmal1^−/− (thick line, black circles) mice. (c) Corresponding mean ± SEM (n=5 per group) rates of insulin secretion calculated as AUC from insulin concentrations obtained during islet perifusion in islets from β-Bmal1^+/+ and β-Bmal1^−/− mice. White bars, 4 mmol/l glucose; black bars, 16 mmol/l glucose. Statistical significance is denoted by *p<0.05