. Author manuscript; available in PMC: 2017 May 1.

Published in final edited form as: Nanotoxicology. 2015 Sep 16;10(4):453–461. doi: 10.3109/17435390.2015.1078852

Table 1.

PCR and sequence primers used in pyrosequencing assay.

Target (Gene ID)	Primer	Sequence (5′–3′)	Annealing temperature (°C)	PCR product (bp)
IFN-γ (15978)	Forward	AATGGTGTGAAGTAAAAGTGTTTTTAGA	53.4	108
	Reverse	AAAATTTCCTTTCCACTCCTTAAACTCTC^a
	Sequencing	ATGGTATAGGTGGGTA
TNF-α (21926)	Forward	GATAATGATTAGTTTTGGAGGATAGAGA	51.9	178
	Reverse	ACACCCAAACATCAAAAAATCT^a
	Sequencing	TGGTTTTAGATTGTTATAGAATT
Thy-1 (21838)	Forward	AAAGAAAGGGTTATAATTTTAAAAGGAGAG^a	52.2	154
	Reverse	ACTACTAAAAAAACACTCTCAATCCTATA
	Sequencing	ACACTCTCAATCCTATAAT

bp, base-pair.

Biotinylated labeled primer.