Figure 6. Functional annotation reveals mechanisms linking differential methylation after ISO to cardiac phenotype.
Cardiac-specific pathways were examined for enrichment in differentially methylated genes using Ingenuity Pathway Analytics, revealing cardiac development to be physiological process most targeted by differential methylation after ISO. Interestingly, several known cardiac genes underwent altered regulation, allowing relationships amongst the signaling network to be mapped. Red nodes are differentially methylated only in BALB/cJ; green nodes only in BUB/BnJ; orange in both and white in neither. Solid lines indicated validated direct relationships; dashed lines, validated indirect interactions.