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. Author manuscript; available in PMC: 2017 Mar 5.
Published in final edited form as: Leuk Res. 2015 Sep 5:S0145-2126(15)30371-4 10.1016/j.leukres.2015.08.017. doi: 10.1016/j.leukres.2015.08.017

Figure 1. Effects of transfected BRAFV600E on the MAPK pathway and cell growth.

Figure 1

A. Immunoblot analysis of wild-type and mutant BRAF expression and MAPK signaling in transfected OSUCLL cells following 24 hr incubation without or with dox (1 µg/ml). B. Proliferation in OSUCLL-Tet, OSUCLL-BRAF, and OSUCLL-BRAFV600E cells without or with dox treatment (1 µg/ml) for 24 hr. BrdU was added for the last 4 hr of the incubations. Data are shown relative to the OSUCLL-Tet control cell line without dox treatment (N=4, *p<0.05; **p<0.005). C. OSUCLL-Tet, OSUCLL-BRAF, and OSUCLL-BRAFV600E cells were incubated 48 hr with or without dox, then stained with anti-CD69 or isotype antibodies and assessed by flow cytometry. Data are representative of three independent experiments.